Joe Cokanasiga and Jamie George kept their places in England side from last week as they take on the Aussies this weekend at Twickenham.
With Nowell and Ashton out with injury Cokanasiga got another shot for this weekend.
It is a surprise that Jones have selected Jamie George infront of his co-captain Dylan Hartley for this weekend.
Tuilangi will also return to the 23 match day squad.
Moon and Sinckler will join George to make up the front-row.
Lawes and Itoje will form the second row for the English with Shields, Underhill and Wilson running out as the loose trio.
“We’ve picked our best 23 for the Australia game,” said Jones. “We know they have a unique style of attack so our ability to defend that is going to be important.
“We are continually working on our strengths which is our set piece and our defence and they are areas that are progressing nicely.
“Australia, we know are a traditional rival and we enjoy the occasion of playing them at Twickenham. The spirit of the fans has been outstanding.
“I think the atmosphere during these Quilter Internationals has been the best atmosphere I’ve certainly experienced and we encourage the fans to be at their robust best at the weekend.”
England: England: 15 Elliot Daly, 14 Joe Cokanasiga, 13 Henry Slade, 12 Ben Te’o, 11 Jonny May, 10 Owen Farrell (c), 9 Ben Youngs, 1 Ben Moon, 2 Jamie George, 3 Kyle Sinckler, 4 Maro Itoje, 5 Courtney Lawes, 6 Brad Shields, 7 Sam Underhill, 8 Mark Wilson
Replacements: 16 Dylan Hartley, 17 Alec Hepburn, 18 Harry Williams, 19 Charlie Ewels, 20 Nathan Hughes, 21 Richard Wigglesworth, 22 George Ford, 23 Manu Tuilagi.